Released on = April 11, 2007, 11:52 am

Press Release Author = AnaSpec Inc.

Industry = Biotech

Press Release Summary = At the 2007 annual conference of the Association of
Biomolecular Resource Facilities, AnaSpec presented a technical poster that compared
the synthesis of a fluorescent peptide using two types of resins.

Press Release Body = April 11, 2007 - Tampa, FL

At the 2007 annual conference of the Association of Biomolecular Resource
Facilities, AnaSpec presented a technical poster that compared the synthesis of a
fluorescent peptide using two types of resins. The results of the poster indicated
that the use of an unprotected resin was just as effective as using a protected
resin for the synthesis of a 5-FAM labeled peptide.

Many FRET (Fluorescent Resonance Energy Transfer) peptides, as well as non-FRET
peptides, requiring C-terminal fluorescent labels can be accomplished using either
solution phase or solid phase synthesis. In solid phase synthesis, the use of the
fluorophore, 5-carboxyfluorescein (5-FAM) generates stable amide bonds. In order to
help facilitate the synthesis of C-terminal fluorescent peptides, AnaSpec prepared
and
performed comparison studies using two kinds of resins, Fmoc-Lys(5-FAM)-Rink Amide
resin (I) and Fmoc-Lys[5-FAM(trt)]-Rink Amide resin (II), the latter contains a
phenolic hydroxyl group protected with a trityl group. The results of these studies
showed that resin (I) is adequate for synthesis of C-terminal fluorescent labeled
peptide.

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